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28.06.2012Funded project for the use of the synthetic potential of P450 monooxygenases
The Federal Ministry of Education and Research (BMBF) has granted a project within the Cluster Industrielle Biotechnologie CLIB2021, in which the synthetic potential of P450 monooxygenases will be investigated by Autodisplay Biotech GmbH and the research group of Prof. Dr. Joachim Jose at the Institute of Pharmaceutical and Medicinal Chemistry at the Westfälische Wilhelms University in Munster.

With the Autodisplay technology, recombinant proteins or peptides can be displayed on the surface of Escherichia coli (E. coli) and thus made available for various biotechnological and biomedical applications. Autodisplay has already been successfully used for the expression of a wide range of different proteins, e.g. hydrolases, esterases, oxygenases and electron transfer proteins. With the help of Autodisplay, recomibnant expressed peptides/proteins are localised in the outer membrane of E. coli allowing even purified outer membrane isolates a number of bioanalytical applications.

The aim of the granted project is the development of a modular screening system for the identification of new synthesis routes through cytochrome P450 monooxygenases (P450). After successfully displaying active P450 enzymes and the still required accessory proteins on the surface of E. coli, the resulting whole cell biocatalysts are to be applied for the identifi-cation of new synthesis routes and thereby improve the biocatalytic potential of the P450 enzymes. "We are convinced that with our technology platform we will be able to make a key contribution to the utilisation of this diverse, but difficult to handle, enzymes." states Dr. Ruth Maas, CEO of Autodisplay Biotech.